Concentration

$$ \frac{meean\ volume \ of\ standard\ solution}{mean\ volume\ of\ fruit\ juice} × concentration\ of\ standard\ solution $$

Safety

• Goggles
• Lab Coat
• Gloves
• Avoid Contact with DCPIP with skin, can irritate and burn skin on contact
  • When vitamin C is added to DCPIP the DCPIP is reduced which caused a colour change from blue to Colourless
  • The concentration of vitamin C in each fruit juice can be calculated by comparing it to the standard solution
  • Transfer with a pipette or a syringe

Vitamin C Test

• Vitamin C is found in citric fruits
• It is essential for a healthy diet
• Vitamin C is a good reducing agent thus easily oxidised
• Methods for the detection of vitamin C involve titrating it against a solution of an oxidising agent called DCPIP
  • DCPIP is a blue dye that turns colourless when vitamin C is added to it

Apparatus

• Vitamin C solutions
• 1% DCPIP solution
• Distilled water
• Range of fruit juices
• Measuring cylinder
• Pipette
• Stop watch