• We know that certain plants release antimicrobials to fight off viruses
• These antimicrobials can be used to produce new drugs for human use

Method

1. Test for different parts of the plant
2. Grind the plant material to make an extract
3. Prepare a lawn of bacteria
4. Use a bunsen burner at a yellow flame to create a convection current to carry away airborne bacteria / Aseptic Technique
5. On an agar petri dish, distribute the bacteria using the streaking method
6. Create 4 equal sized wells (opening in the gel)
7. Place the paper discs soaked with different plant extracts, and the fourth being soaked with water as a control experiment
8. Seal the lid at both sides but not all the way using cello-tape, to prevent anaerobic conditions, that might kill the bacteria
9. Incubate at temperatures 20-35 for 24 hours to 7 days, to prevent the growth of pathogenic organisms
10. Measure the diameter of the clear zone around each disc of inhibitor
11. Put the petri dish in a plastic back for disposal, seal and sterile at 121 C for 15 min under high pressure and temperature then throw away
12. Repeat and calculate the mean

Aseptic Technique

1. Complete the procedure with a bunsen burner at yellow flame to kill any airborne microorganisms by creating a convection current
2. When opening the lid of the petri dish, allow slight degree of opening to avoid contamination with another microorganism causing disease